Handbook add request: RNA extraction protocol #2526

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1. sr320 commented on Oct 20, 2026

   

   sr320

   on Oct 20, 2026 via email

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   Not exactly what you are asking.. but one of those things you should know - https://robertslab.github.io/tusk/framework/gene-expression-methylation.html

   …

   On Tue, Oct 20, 2026 at 2:55 PM Megan Ewing ***@***.***> wrote: *meganewing* created an issue (RobertsLab/resources#2526) <https://urldefense.com/v3/__https://github.com/RobertsLab/resources/issues/2526__;!!K-Hz7m0Vt54!gKnp_VsRhI_kcyJbuqYivyl76PTJs48y54HuGC6yaVqDVs0uxETdy2AofU6gdqyeSZULca875aPBnMYowskLtBc$> Ie. how to go from Animal to cDNA to be used for the qPCR workflow <https://urldefense.com/v3/__https://robertslab.github.io/resources/protocols/protocol-qPCR/*part-2-bench-protocol-sop__;Iw!!K-Hz7m0Vt54!gKnp_VsRhI_kcyJbuqYivyl76PTJs48y54HuGC6yaVqDVs0uxETdy2AofU6gdqyeSZULca875aPBnMYoj91k5tg$> — Reply to this email directly, view it on GitHub <https://urldefense.com/v3/__https://github.com/RobertsLab/resources/issues/2526?email_source=notifications&email_token=ABB4PN65L4VXIBLV7HAJIQT5U7NNHA5CNFSL4Z3JMQ5C6L3HNF2C22DVMIXUS43TOVSS6NJZGUYDEOJQHE2DPJTSMVQXG33OVJZXKYTTMNZGSYTFMSSWK5TFNZ2KYZTPN52GK4S7MNWGSY3L__;!!K-Hz7m0Vt54!gKnp_VsRhI_kcyJbuqYivyl76PTJs48y54HuGC6yaVqDVs0uxETdy2AofU6gdqyeSZULca875aPBnMYo_IWe12k$>, or unsubscribe <https://urldefense.com/v3/__https://github.com/notifications/unsubscribe-auth/ABB4PNZ7I5R3URAHKIRZSET5U7NNHAVCNFSNUABEKJSXA33TNF2G64TZHM4TSNRZHA3DQOB3JFZXG5LFHM2TSNJQGI4TAOJUG6QXMAQ__;!!K-Hz7m0Vt54!gKnp_VsRhI_kcyJbuqYivyl76PTJs48y54HuGC6yaVqDVs0uxETdy2AofU6gdqyeSZULca875aPBnMYoNOUd0Ao$> . You are receiving this because you are subscribed to this thread.Message ID: ***@***.***>

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2. kubu4 commented on Oct 20, 2026

   

   kubu4

   on Oct 20, 2026

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   This is tough. There are many methods available/used by the lab, so it's not something that is really defined.

   But, basically, the most common approach is this:

   • Use RNA isolation kit and follow the kit protocol. 😃

   Tissue homogenization is also variable. Can use LN2 and mortart/pestles (best, but most labor intensive), Bullet Blender (currently the highest throughput and easiest labor), disposable mortar/pestles (good, but lab intensive - less so if using powered homogenization adaptor).

   So, a write up on all of this could be a bit too lengthy, leading to confusion.

   I guess we could link out to different pages describing each of the homogenization methods?
3. meganewing commented on Oct 20, 2026

   

   meganewing

   on Oct 20, 2026

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   Ah. Given that, I think even just adding a parenthetical link in the qPCR or RT handbook page to 1-2 examples of a RNA isolation kit's (perhaps the most common used 1-2 in the lab?) manufacturer protocol page would be helpful?
4. kubu4 commented on Oct 23, 2026

   

   kubu4

   on Oct 23, 2026

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   I've taken some steps to address this:

   1. Added a link to TUSK in the Handbook: https://robertslab.github.io/resources/tusk/
   2. Added a Lab Work page in TUSK, with some rough guidelines to RNA isolation procedures: https://robertslab.github.io/tusk/modules/06-lab-work.html
5. meganewing commented on Oct 23, 2026

   

   meganewing

   on Oct 23, 2026

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   @kubu4 This rocks!!!! thanks Sam!!
6. kubu4

   closed this as completedon Oct 29, 2026